Ukuvala inzalo kubhekisela ekusetshenzisweni kwezici eziqinile zomzimba namakhemikhali ukwenza zonke izinambuzane ngaphakathi nangaphandle kwanoma iyiphi into zilahlekelwe ukukhula nekhono lokuzala unomphela.Izindlela ezisetshenziswa kakhulu zokuvala inzalo zifaka ukuvala inzalo ngamakhemikhali, ukuvala inzalo ngemisebe, ukuvala inzalo yokushisa okomile, ukuvalwa kokushisa okumanzi kanye nokuvalwa kokuhlunga.Izindlela ezahlukene zingasetshenziswa ngokwezidingo ezahlukene.Isibonelo, i-medium ivalwa ukushisa okumanzi, futhi umoya uhlanzwa ngokuhlunga.
Ilambu lensimbi engenasici liyisibani semercury esinengcindezi ephansi.Isibani se-mercury esinengcindezi ephansi sikhipha ukukhanya kwe-ultraviolet ngokujatshuliswa umfutho ophansi we-mercury vapor (<10-2Pa).Kunemigqa emibili eyinhloko yokukhipha i-spectral: eyodwa ingu-253.7nm wavelength;enye ingu-185nm wavelength, womabili angamehlo anqunu Imisebe ye-ultraviolet engabonakali.Isibani sensimbi engagqwali esibulala amagciwane asidingi ukushintshwa sibe ukukhanya okubonakalayo, futhi ubude begagasi obungu-253.7nm bungadlala umphumela omuhle wokuvala inzalo.Lokhu kungenxa yokuthi amaseli anokujwayelekile kububanzi bokumuncwa kwamagagasi okukhanya.Imisebe ye-Ultraviolet eku-250~270nm inokumunca okukhulu futhi iyamuncwa.Ukukhanya kwe-ultraviolet empeleni kusebenza kuzakhi zofuzo zengqamuzana, okuyi-DNA.Idlala uhlobo lomphumela we-actinic.Amandla e-ultraviolet photon amuncwa amapheya ayisisekelo ku-DNA, okubangela ukuthi izakhi zofuzo ziguquke, okubangele ukuthi amagciwane afe ngokushesha noma angakwazi ukuzala kabusha inzalo yawo.Ukufeza inhloso yokuvala inzalo.